Pure TMG is a Methyl donor supplement by DoNotAge. NO1GEVITY gives it a overall score of 6.7 out of 10.

How much does Pure TMG cost?

$30.00 for 30 servings. Cost per day at the studied dose is $1.50.

Is Pure TMG tested by independent lab?

Yes, tested by independent lab. Certificate of Analysis is publicly available.

What is the NO1GEVITY score for Pure TMG?

Pure TMG scores 6.7/10 on the NO1GEVITY composite scale, based on evidence-graded dose (100% of trial dose), transparency, value, consumer signals, and formulation.

Does Pure TMG match the dose used in clinical studies?

Pure TMG delivers 100% of the dose used in clinical research, which is at or above the studied dose.

What is the evidence for Betaine (TMG)?

Betaine (TMG) is graded C on the NO1GEVITY evidence scale, meaning limited human evidence, mostly small trials or pilot studies.

Layer 1 · Decision

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DoNotAge

Pure TMG

Name: Pure TMG
Brand: DoNotAge
SKU: donotage-tmg
Price: 30.0 USD
Availability: InStock

Methyl donor · Powder · 30 servings

Label snapshot

What the label says

What the product contains and what one serving costs, according to our catalog. Label amounts are not personal dose recommendations.

Active per serving: Betaine (TMG) 2,000 mg
Form: Powder
List price (catalog, USD): $30.00
Price per serving: $1.00List price divided by servings per container. A per-day cost only if the label serving is once daily.

Full label record: servings, testing, ingredients

Servings per container: 30
Serving size (label): 2 g
Catalog monthly estimate: $30.00
Testing disclosure: Brand lists third-party testing and a public certificate of analysis (CoA)Status as recorded in the catalog from brand materials; not independently verified by NO1GEVITY.

Ingredients as listed

Ingredient	Amount per serving
Betaine (TMG)	2,000 mg

What to check on the label

Whether the label says betaine anhydrous (TMG) or betaine HCl. They are different products.
For folate, the form named (5-MTHF, methylfolate, folic acid) and the microgram amount.

Amounts are per label serving as recorded in the catalog and are not a recommendation. Testing status reflects what the brand lists. Nothing here is medical advice.

Editorial score and research-dose calculations

These calculations describe our scoring method. They are not a recommended dose or proof of benefit from this product.

6.7/10

Overall score

#2 / 5 in Methyl donor

Public lab certificate, tested by independent lab.

Read the evidence (3 studies)

Add to compare Visit brand site →

Tier 3

Ingredient RCT (matching dose)

The ingredient was tested in an RCT at the same dose this product sells.

How we scored this product Show the full breakdown

Dose vs. studies (50%) 5.4

Transparency (20%) 9.5

Value (10%) 8.0

Formulation (10%) 5.0 Default. No absorption data on file.

Safety (5%) 8.5

Community sentiment (5%) 8.2

Composite 6.7

6.7 composite

Dose vs. studies50%5.4

Transparency20%9.5

Value10%8.0

Formulation10%5.0

Safety5%8.5

Community5%8.2

Evidence gates dose: the evidence grade sets the ceiling, and the dose decides how much of that ceiling the product reaches. Value = inverse of price-per-trial-dose-day. Community = mean sentiment of external reviews. Safety caps applied after weighting.

This score is our editorial judgment, not a scientific standard. We combine published evidence, dose, price, transparency, and consumer signals into one composite: this is our method, not a peer-reviewed clinical rating. A higher score means a product does better on the factors we weight, not that it is medically proven to work.

Read the full method →

Best for

Full trial dose, Public lab certificate

Avoid if

No major red flags

Full-dose status

Meets trial dose

Price/month

$30

COA status

Public

Biggest caveat

Dose-evidence score of 5.4 is below average for the site.

Nearest alternatives

NiaCel 400 Thorne HealthTech · Score 7.7
Nicotinamide Riboside Pro 300 + 100mg TMG ProHealth Longevity · Score 7.5
NiaCel 200 Thorne HealthTech · Score 7.0

Catalog snapshot. Check linked sources for current price, label and batch-specific testing.

Price verification

Current price: $30.00

Last price check: Sep 01, 2026

Add to compare Visit brand site →

Layer 2 · Context

Our summary

Pure TMG (trimethylglycine/betaine) is DoNotAge's methyl-donor companion product, marketed specifically to be stacked with high-dose NAD+ precursors like Pure NMN to offset methylation demand — a legitimate physiological rationale, since NAD+ precursor metabolism does consume methyl groups. Of every product in this dossier, TMG has the cleanest, most directly dose-matched human evidence: a controlled trial tested exactly the 1000 mg/day dose DoNotAge markets and found it reliably lowers homocysteine, a recognized cardiovascular risk marker. Liver-health and cognitive claims on the product page, however, are not backed by specific cited studies at this dose.

In plain language

Why this scored this way

This DoNotAge Pure TMG scores 6.7, making it a mid-tier product with both strengths and weaknesses. It delivers 2000mg of Betaine (TMG), inside the studied range of 1500–6000mg with grade C evidence (mostly preclinical, limited human data). Transparency is a clear strength (score: 9.5), with third-party testing and a public lab certificate. At $1.50/day it's among the more expensive options in Methyl donor.

What drives the score up

Quality transparency of 9.5: tested by independent lab with a public lab certificate.
Community score of 8.2 with positive consumer feedback.

What drags it down

Dose-evidence score of 5.4 is below average for the site.

Why you'd choose it

You want a reasonably priced methyl donor product.
You prioritise tested by independent lab products with public lab certificates.

Why you might skip it

You want better value (this is $1.50/day with a value score of 8.0).

Question 1

Does the molecule work?

The science behind each active ingredient, independent of which brand sells it.

Betaine (TMG)

A methyl donor added to NAD+ formulas as a supporting cofactor, not a headline active.

No landmark RCTs listed · US: Dietary supplement, GRAS · EU: Food supplement, permitted

Question 2

Is this bottle good?

The specific product: dose, price, transparency, and what buyers report.

Dose vs research

100% of trial dose

Meets or exceeds the published effective dose

Cost at trial dose

$1.50/day at trial dose

What one effective daily dose actually costs

Transparency

9.5/10 · Public lab certificate (quality: not assessed)

Independent batch testing confirmed

Consumer signals

No consumer signals collected yet

Operational signal only, never used as clinical evidence

Question 3

What's the catch?

Safety flags, transparency gaps, dosing concerns, and recurring buyer complaints.

No major red flags

Public lab certificate, tested by independent lab, dose within range

No safety, transparency, or dose concerns detected at this time.

Plain English

What’s inside

The main ingredients of this product, in plain language.

Betaine (TMG)2000 mgEvidence C

A methyl donor added to NAD+ formulas as a supporting cofactor, not a headline active.

Read about Betaine (TMG) →

Layer 3 · Evidence vault

Product data & verification

Hard numbers and independently checked status. Expand each block for its source.

The numbers

Price

$30

per bottle · 30 servings

Dose vs. research

of trial dose 100%

of published effective dose

$ / day at studied dose

$1.50

what one effective daily dose costs

$ / g active

$0.50

per gram of active substance

Independently checked

Data verification

COA status

Public

Evidence grade

Per-ingredient breakdown

CBetaine (TMG)

All product data is checked against original sources. Prices, doses, and testing status are updated when product pages change. Evidence grades are reviewed when new human RCTs are published. Read how we verify.

What's inside

Ingredient

Dose in this product

Trial dose

Evidence

Betaine (TMG)

A methyl donor added to NAD+ formulas as a supporting cofactor, not a headline active.

2000 mg 67% of trial dose

3000 mg

4.1 /5

Medium confidence

556 reviews across 2 platforms (2 sources)

Source diversity: 46%

RedditTrustpilot

PraiseFrequently paired with NMN/NR to offset methyl-donor depletion. DoNotAge is a co

Last checked: 2026-08-07

Consumer signals

Consumer signals gathered from 2 platforms.

Low confidence, small sample

Consumer sentiment compiled from 1 public source (Reddit). Small sample size, treat as directional only.

1 thread discussed · positive sentiment

Praise

Frequently paired with NMN/NR to offset methyl-donor depletion. DoNotAge is a common community pick.

Compiled from a Reddit r/longevity listing located during manual research; not independently re-verified against the live page at build time.

Trustpilot

556 reviews · 4.1/5

These are consumer sentiment signals. They do not measure whether the product actually works. See our evidence section for that.

What others say, with a source hierarchy

We treat external opinion very differently depending on where it comes from. Tier A is our strongest signal. Tier C is background sentiment only. Tier Rejected is what we excluded, listed openly so you can see what we discarded.

These reviews are aggregated from third-party sources, not written by NO1GEVITY. Each entry shows its source, retrieval date, and whether the source has an affiliate or paid relationship. We do not edit reviews to change their meaning. Disclosure policy.

Tier B

Registered dietitians and physicians, without affiliate ties

Credentialed commentary from clinicians who disclose no paid relationship. Useful as supporting critique but not a substitute for a clinical outcome trial.

Physician YouTube analysis — DoNotAge Longevity Sachet

Has affiliate relationship Not a paid placement

Detailed ingredient walk-through. Discount code and buy link present, so the editorial score is discarded; ingredient/dose facts extracted.

How we use it: Ingredient/dose facts only. Editorial rating rejected.

Tier C

Aggregated sentiment (Trustpilot, Reddit)

Mood indicator only. We never use aggregate stars as evidence of efficacy.

Trustpilot — DoNotAge (aggregated sentiment)

No affiliate relationship Not a paid placement

Aggregate score ≈ 4.3-4.5 / 5. Mood indicator only.

Finding: 4.3-4.5 / 5.

How we use it: Sentiment only. Never used as evidence of efficacy.

Community threads and aggregate ratings

Additional public mentions we located, kept separate from the deep independent reviews above.

Reddit r/longevity

positive

Frequently paired with NMN/NR to offset methyl-donor depletion. DoNotAge is a common community pick.

Read source → Unverified · we received no payment for this listing

Layer 3 · Evidence vault

Evidence, claims & regulation

Published evidence, claim checks, regulatory status and what could not be verified.

Evidence, what actually backs this product

Longevity brands rarely test their exact finished product. We separate what's tested from what's inferred.

Strongest evidence tier

Tier 3Ingredient RCT (matching dose)

3 studies mapped

Tier 3Ingredient RCT (matching dose)

Low dose betaine supplementation leads to immediate and long term lowering of plasma homocysteine in healthy men and women

Steenge GR, Verhoef P, Katan MB · 2003 · Journal of Nutrition · 1000 mg/day (and other doses tested) · 6 weeks

ingredient-levelpeer-reviewed

This controlled trial found that 1000 mg/day betaine supplementation raised steady-state serum betaine substantially (from roughly 31 to 52 micromol/L, about 67%) and produced a measurable, lasting reduction in plasma homocysteine, directly matching DoNotAge's marketed dose.

One of the strongest dose-matches in this whole dossier: the exact 1000 mg/day dose DoNotAge markets was directly tested in healthy adults and found effective at lowering homocysteine, a genuine cardiovascular risk marker.

Conflicts: Academic study (Wageningen University); no disclosed link to DoNotAge.

Source →

Tier 3Ingredient RCT (matching dose)

Betaine supplementation decreases plasma homocysteine in healthy adult participants: a meta-analysis

Various (systematic review authors) · 2013 · peer-reviewed meta-analysis (PMC-indexed) · Various, generally up to 6000 mg/day across pooled trials · Varies by pooled trial

ingredient-levelpeer-reviewed

This meta-analysis confirmed that betaine supplementation reliably lowers plasma homocysteine by roughly 5-20% in healthy adults across a range of doses, reinforcing the consistency of this specific biomarker effect.

Strengthens confidence in the homocysteine-lowering claim generally, though it does not test cognitive or liver-health claims.

Conflicts: Academic meta-analysis.

Source →

Tier 4Ingredient RCT (different dose)

Betaine supplementation decreases plasma homocysteine concentrations but does not affect body weight, body composition, or resting energy expenditure

Schwab U, Törrönen A, Toppinen L, et al. · 2002 · American Journal of Clinical Nutrition · 6000 mg/day · 12 weeks

ingredient-levelpeer-reviewed

At a much higher dose than DoNotAge's product (6 grams vs 1 gram), betaine again lowered plasma homocysteine but had no effect on body weight, body composition, or metabolic rate — useful for confirming the homocysteine effect but not for any metabolic/weight claim.

Included to show the homocysteine-lowering effect replicates across a range of doses, but this specific trial used 6x DoNotAge's dose and is not directly dose-matched.

Conflicts: Academic study; no disclosed link to DoNotAge.

Source →

Evidence

Research behind this product

No landmark studies linked yet. See the ingredient pages for the full evidence breakdown.

Claim check, vendor claims vs. our reading of the evidence

Not medical advice. We list the strongest claims made by the brand and check each against traceable evidence.

Supported

“Balances homocysteine, supporting heart health and vascular function”

This is the best-supported claim across the entire DoNotAge product range in this dossier: a dose-matched RCT (Steenge 2003) at exactly 1000 mg/day, backed by additional trials at other doses and a meta-analysis, consistently show betaine lowers plasma homocysteine, a recognized cardiovascular risk marker.

Vendor source

Partial support

“Promotes liver health, encouraging detoxification and fat metabolism”

Betaine has documented lipotropic (fat-metabolizing) effects in liver physiology and has been studied in non-alcoholic fatty liver disease contexts in some research, but we did not locate a specific human RCT at DoNotAge's marketed dose directly testing a 'liver health' outcome to fully substantiate this specific framing.

Vendor source

No evidence

“Enhances cognitive function via neurotransmitter production”

No specific cognitive-outcome trial was located in the literature we reviewed for betaine/TMG at this dose; the ingredient's core, well-evidenced role is as a methyl donor affecting homocysteine metabolism, not a direct cognitive enhancer.

Vendor source

Regulatory status

Rules change often. We record when we last verified each row. See the full regulatory timeline →

Legal as supplement

Betaine/TMG is a long-established, GRAS-recognized dietary ingredient in the US with no restriction comparable to NMN.

Last verified 2026-08-05

Legal as supplement

Betaine has an established history of use as a food and supplement ingredient in the EU and is not subject to Novel Food restrictions.

Last verified 2026-08-05

Legal

Sold as a standard food supplement ingredient in the UK.

Last verified 2026-08-05

Legal as supplement

Betaine/TMG is commonly licensed as a Natural Health Product ingredient in Canada; specific DoNotAge NPN status was not confirmed.

Last verified 2026-08-05

What we could not verify

We list this openly. If a claim isn't backed by a source we can trace, we'd rather flag it than repeat it.

The exact per-capsule milligram amount (as opposed to the marketed 'per serving' total) was not confirmed from the pages we could access, since the product page itself timed out during retrieval and details were sourced from secondary listings.
The liver-health and cognitive-function claims could not be traced to specific supporting human trials.

Important to know

Betaine (TMG)Doses under roughly 500 mg/day (as seen in most NAD+ combination products) are well below the gram-level doses used in the supporting human trials.

Editorial notes

Companion to NAD+ boosters to offset methyl depletion.

Go deeper

Where this sits

Actives inside

Betaine (TMG)

Spot an error?

If you are the manufacturer or brand owner of Pure TMG and believe any data on this page is incorrect (wrong dose, wrong ingredient, wrong price, missing certification, outdated status), you can request a correction.

We review every request, verify it against primary sources, and publish any accepted change with a dated note. Corrections from brand owners are treated the same as corrections from anyone else: verified before they move.

Request a correction →

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Pure TMG

What the label says

Ingredients as listed

What to check on the label

Quick verdict

Our summary

Why this scored this way

What drives the score up

What drags it down

Why you'd choose it

Why you might skip it

Does the molecule work?

Is this bottle good?

What's the catch?

What’s inside

Product data & verification

The numbers

Data verification

What's inside

Consumer signals

What others say, with a source hierarchy

Registered dietitians and physicians, without affiliate ties

Aggregated sentiment (Trustpilot, Reddit)

Community threads and aggregate ratings

Evidence, claims & regulation

Evidence, what actually backs this product

Research behind this product

Claim check, vendor claims vs. our reading of the evidence

Regulatory status

What we could not verify

Editorial notes

Where this sits

Spot an error?

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